Coding

Part:BBa_K2926068:Design

Designed by: Johanna Opgenoorth   Group: iGEM19_Bielefeld-CeBiTec   (2019-10-15)


Proline-glycine-peptide fused to the n-terminus of mCherry


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

To enable the interaction of the n-terminal proline with its target transporter we designed a short sequence of prolines and glycines to minimize sterical hindrance and formation of secondary structures within the n-terminus.


Source

The proline-peptide was fused via add on PCR to the iGEM part BBa_J06504.

References